Composite

Part:BBa_K3196032:Design

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


pGAP-Kozak-PHO1-pelA-His tag-TT-Pgpda-Kozak-FLO10-SLAC-His tag-TT-Pgas-Alkali-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BamHI site found at 478
    Illegal BamHI site found at 2127
    Illegal BamHI site found at 2138
    Illegal BamHI site found at 2149
    Illegal BamHI site found at 3501
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2588
    Illegal SapI site found at 238


Design Notes

d


Source

d

References